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Saturday, May 28, 2022

Sure, SARS-CoV-2 Is a Actual Virus

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Whereas some nonetheless declare SARS-CoV-2 doesn’t really exist, this appears to fly within the face of a number of well-established information. The virus has really been photomicrographed,1,2 whole-genome sequences of the assorted strains can be found,3,4 and with the suitable credentials anybody can get hold of the dwell virus to conduct analysis.

Whereas I’m completely no fan of the U.S. Facilities for Illness Management and Prevention, they do develop the virus in cell tradition to make sure widespread availability for researchers who need to examine it.5 Examples of analysis the place you want the precise virus embrace antiviral analysis, vaccine improvement, virus stability analysis and pathogenesis analysis.6

What’s the Confusion?

Not less than a part of the confusion seems to be rooted in how the time period “remoted” is outlined. Some insist a virus shouldn’t be remoted until it’s additionally purified, whereas others say a virus doesn’t should be purified with a view to be “remoted.”

Steve Kirsch claims to have requested a number of specialists about this, noting that every one, together with Dr. Robert Malone and Dr. Li-Meng Yan, say that the virus has certainly been “remoted.” “So, it has been ‘remoted’ in accordance with their perception in what the time period means,” Kirsch writes, including:7

“Others interpret the time period otherwise and would declare the virus hasn’t been remoted. In truth, in accordance with their definition, no virus in historical past has ever been remoted. That’s necessary to know. They use that as justification for his or her perception that there isn’t a virus right here since viruses don’t exist in any respect.”

When Kirsch requested his readers for enter, one identified:8

“The actual query is … has it been remoted from a HUMAN topic w/o passing it by means of (say) Monkey Kidney Cells? As a result of there’s loads of proof on the market that claims it hasn’t been remoted instantly (no intermediaries) from a HUMAN topic.”

In response to Kirsch, the scientists he spoke with didn’t agree that this was a priority, and “Sabine Hazan verified that the sequence of the virus obtained from ATCC [the American Type Culture Collection, a global resource center for reference microorganisms] matched precisely what she present in individuals who have the virus.”9

As famous in Hazan’s paper, “Detection of SARS-CoV-2 From Affected person Fecal Samples by Complete Genome Sequencing”:10

“Research members underwent testing for SARS-CoV-2 from fecal samples by entire genome enrichment NGS [next-generation sequencing] (n = 14), and RT-PCR nasopharyngeal swab evaluation (n = 12).

The concordance of SARS-CoV-2 detection by enrichment NGS from stools with RT-PCR nasopharyngeal evaluation was 100%. Distinctive variants have been recognized in 4 sufferers, with a complete of 33 completely different mutations amongst these by which SARS-CoV-2 was detected by entire genome enrichment NGS.”

Germ Idea and Terrain Idea Each Have Advantage

As famous by impartial journalist and political analyst Jeremy Hammond in a March 2021 interview,11 the declare that SARS-CoV-2 has by no means been remoted and truly doesn’t exist in any respect is probably one of the vital counterproductive arguments of the well being freedom motion.

By insisting that there isn’t a virus, and that COVID-19 is brought on by issues like 5G radiation alone, permits the mainstream media to dismiss fully legit considerations about electromagnetic area publicity (EMF) and 5G — together with the chance that it would make some folks extra susceptible to infections.

Like Hammond, I consider the pathogenesis of COVID-19 entails each germ idea and terrain idea, not only one or the opposite. “SARS-CoV-2 an infection is an inadequate however essential issue within the pathogenesis of COVID-19,” Hammond says, including that “the virus is consistently being remoted and entire genome sequenced by scientists all around the world.”12

That stated, environmental elements can clearly play a task, in that they will make you roughly predisposed to extreme an infection once you encounter this virus. This contains EMFs, toxins like glyphosate, earlier vaccine accidents and far more.

Hammond argues that the “COVID-19 pandemic ought to be a wake-up name to the human inhabitants, and particularly the populations of developed international locations, about the necessity to deal with pure technique of sustaining good well being and residing in better concord with our pure setting.”

Certainly. And, as Hammond factors out, pathogenic problem is completely essential for normal good well being and robust immunity. Once we protect ourselves an excessive amount of from on a regular basis pathogens, we make ourselves susceptible to power illnesses as an alternative.

SARS-CoV-2 Genome Sequencing From Italy

As for whether or not SARS-CoV-2 has been remoted and exists as a viral entity, the reply seems to be sure. For instance, an Italian paper13 revealed within the Journal of Virology, dated Could 18, 2020, detailed the isolation and full-length genome of the virus taken from COVID-19 sufferers in Italy:

“In the beginning of March 2020, the primary nasopharyngeal swabs constructive for SARS-CoV-2 began to be detected within the Northern Japanese Area of Friuli-Venezia Giulia … Swab contents have been seeded on Vero E6 cells and monitored for cytopathic impact and by an RT-PCR protocol utilizing primers for the N area.

Cell tradition supernatants from passage 1 (P1) of 4 isolates have been collected, and RNA was extracted with QIAamp viral RNA minikit (Qiagen) and quantified with an in vitro-transcribed RNA customary … The amount and high quality of the RNA have been assessed … For every pattern, 100 ng of complete RNA was processed utilizing Zymo-Seq RiboFree ribosomal depletion library preparation package (Zymo Analysis).

All of the obtained libraries handed high quality verify and have been quantified earlier than being pooled at equimolar focus and sequenced … Sequenced reads that handed the standard verify (Phred rating ≥30) have been adaptor and high quality trimmed, and the remaining reads have been assembled de novo utilizing Megahit (v.1.2.9) with default parameter settings.

Megahit generated in all instances 7 contigs with greater than 1,000 bp and 100× protection; all of those assembled contigs have been in contrast (utilizing BLASTn) towards all the nonredundant (nr) nucleotide and protein databases.

In all instances the longest and extra lined contigs have been recognized as MT019532.1,14 ‘Extreme acute respiratory syndrome coronavirus 2 isolate BetaCoV/Wuhan/IPBCAMS-WH-04/2019, full genome,’ with 99% identification and 0 gaps.

The longer sequences have been named hCoV-19/Italy/FVG/ICGEB_S1, _S5, _S8, and _S9 and have been deposited in GISAID … Sequence evaluation confirmed an uneven protection alongside the SARS-CoV-2 genome, with a median vary from 126 to 7,576 reads and a imply protection per pattern of 1,169× … Phylogenetic bushes have been inferred utilizing the utmost chance methodology …

The primary sequences deposited in GISAID (EPI_ISL_410545 and EPI_ISL_410546) have been collected in Rome from a Chinese language vacationer from Hubei province who acquired contaminated earlier than visiting Italy, and one other one (EPI_ISL_412974) was from a test-positive Italian citizen coming back from China.

Solely two sequences have been reported from the Lombardy cluster (EPI_ISL_412973 and EPI_ISL_413489). On this report 4 further sequences from instances epidemiologically linked to northern Italy have been examined … Sequence evaluation confirmed protection alongside the SARS-CoV-2 genome for all 4 isolates.

Primarily based on the marker variant S D614G, all 4 sequences grouped within the Bavarian rooted subclade G, which is dominant in Europe, together with the sequence from Lombardy, however distinct from the three sequences talked about above originating instantly from China.

Intriguingly, the brand new isolates have been extra carefully associated to EPI_ISL_412973, whereas EPI_ISL_413489 was extra distant. No proof might be discovered for the putative 382-nucleotide (nt) deletion in ORF8 detected in Singapore, which has been proposed to point an attenuated phenotype.”

SARS-CoV-2 Genome Sequencing From Germany

Equally, the entire genome sequence of the virus taken from a German girl has been revealed, this one within the journal Microbiology Useful resource Bulletins, in June 2020.

Right here, an oropharyngeal swab pattern from a feminine affected person who examined constructive however had no signs on the time of the take a look at was used to isolate the pressure.15 Desk 1 within the paper compares the nucleotide variants discovered within the sampled virus and people of a reference pressure already logged within the gene financial institution.

One other paper16 in Annals of Inner Medication, revealed in August 2020, remoted the virus from ocular (eye) secretions of an Italian COVID affected person:17

“The affected person, a 65-year-old girl, travelled from Wuhan, China, to Italy on 23 January 2020 and was admitted on 29 January 2020, 1 day after symptom onset. At admission to the excessive isolation unit … she offered with nonproductive cough, sore throat, coryza, and bilateral conjunctivitis. She had no fever till day 4, when fever (38 °C), nausea, and vomiting started.

An infection with SARS-CoV-2 was confirmed by performing real-time reverse transcription polymerase chain response (RT-PCR) assay on sputum samples (cycle threshold worth [Ct], 16.1) on the admission day, adopted by viral M gene sequencing (GenBank accession quantity MT008022), and virus isolation on Vero E6 cell line (2019-nCoV/Italy-INMI1).

The total genome sequence was obtained from both medical pattern or tradition isolate (GISAID accession numbers EPI_ISL_410545 and EPI_ISL_410546).”

Genome Sequencing From India and Colombia

SARS-CoV-2 has additionally been remoted from the urine of a COVID-19 affected person.18 A November 2020 paper19 sought to find out “whether or not numerous medical specimens obtained from COVID-19 sufferers include the infectious virus,” and located SARS-CoV-2 RNA “in all naso/oropharyngeal swabs and saliva, urine and stool samples collected between Days 8 and 30 of the medical course.”

Viable SARS-CoV-2 was additionally discovered within the nasal washes of ferrets that had been inoculated with urine or stool from a COVID-19 affected person. The virus has additionally been remoted by researchers within the U.S.,20 China,21 India,22 Canada,23 Australia,24 Korea25 and Colombia.26 The Colombian paper reads partially:27

“Goal: To explain the isolation and characterization of an early SARS-CoV-2 isolate from the epidemic in Colombia. Supplies and strategies: A nasopharyngeal specimen from a COVID-19 constructive affected person was inoculated on completely different cell strains.

To verify the presence of SARS-CoV-2 on cultures we used qRT-PCR, oblique immunofluorescence assay, transmission and scanning electron microscopy, and next-generation sequencing.

Outcomes: We decided the isolation of SARS-CoV-2 in Vero-E6 cells by the looks of the cytopathic impact three days post-infection and confirmed it by the constructive ends in the qRT-PCR and the immunofluorescence with convalescent serum.

Transmission and scanning electron microscopy photographs obtained from contaminated cells confirmed the presence of constructions appropriate with SARS-CoV-2. Lastly, an entire genome sequence obtained by next-generation sequencing allowed classifying the isolate as B.1.5 lineage.

The proof offered on this article confirms the primary isolation of SARSCoV-2 in Colombia. As well as, it exhibits that this pressure behaves in cell tradition in an analogous strategy to that reported within the literature for different isolates and that its genetic composition is in keeping with the predominant variant on the earth.”

If Virus Exists, Why Aren’t Sure Research Performed?

As talked about earlier, the precise virus is required with a view to conduct sure research. Now, for the reason that virus does exist, we additionally ought to have the ability to conduct research to evaluate whether or not the COVID pictures trigger antibody dependent enhancement (ADE).

As steered by Kirsch,28 “Give the vaccine to the animals, wait, then expose them to the virus” and see what occurs. Does it forestall an infection and transmission, or does it make the animals extra susceptible to an infection? If the animals acquired sicker, that will be proof of ADE, an issue that has plagued coronavirus vaccine analysis for many years.

It’s why we don’t have a vaccine towards the frequent chilly, brought on by coronaviruses. Remarkably, this animal analysis has by no means been performed for the COVID pictures. The query is why? Kirsch believes the reply is as a result of “no one desires to know the reply … The highest administration of the FDA is aware of it will kill the vaccine program in the event that they did this.”

Alternatively, the vaccinated, similar to the unvaccinated, are likely to expertise solely gentle signs with Omicron. So, maybe the pictures aren’t inflicting ADE (which may flip even a milder variant into one thing lethal).

Nevertheless, ADE is way from the one concern. Clearly, these pictures are related to a dramatically elevated danger of cardiovascular, cardiac and neurological issues. These too might be confirmed by means of animal research — relatively than testing on our youngsters — and we wouldn’t even want the virus for these.

Both means, I consider it’s scientifically correct to say that SARS-CoV-2 has been remoted, genetically sequenced, and that it exists as a pathogenic entity. Getting too far into the weeds of theories that refute the existence of viruses altogether will solely decelerate and hamper the reality motion relatively than assist it alongside, and I might strongly discourage anybody from participating on this extremely unproductive narrative.

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